Hemoglobin Biosynthesis in the Chicken Red Blood Cells and Inhibitory Effect of Actinomycin D*

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Abstract
The incorporation of Cl4-L-valine into hemoglobin by intact erythrocytes of the adult chicken, and into total protein by a cell-free system, consisting of microsomes and L-amino-acid: sRNA ligase fraction, was carried out. The incorporating activity of intact cells from normal erythrocytes is very low as compared with reticulocytes. Puromycin almost completely inhibited this incorporation. The similar magnitude of incorporation was observed in the 2 main components of the hemoglobin fraction. Actinomycin D inhibits the incorporation into hemoglobin and the other cellular protein components by intact reticulocytes, especially at the later part of the incubation. The incorporating activity of the cell-free system, consisting of microsomes and the L-amino-acid: sRNA ligase fraction from normal erythrocytes, is very low as compared with that from reticulocytes. The incorporation is dependent on the energy-generating system and is inhibited almost completely by puromycin. Actinomycin D has no effect on the incorporation. Discussion of these results was made on the relationship between RNA biosynthesis and hemoglobin biosynthesis.