Growth and Spore Production of Clostridium botulinum Type E in Chemically Defined Media

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Abstract
A chemically defined medium is described for the growth and spore production of Clostridium botulinum type E, strain Minnesota. With the exception of valine, omitting any amino acid or vitamin did not prevent growth, although marked changes in morphology or reduced growth were usually evident. Choline was shown to be an essential factor in promoting cell division and was capable of exerting this action after maximum growth was attained. Spore development in the medium was markedly influenced by the source of available carbon and ranged from complete sporulation of cultures grown on maltose and glucose to complete inhibition with galactose.

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