Prostaglandin F2α stimulates phosphatidylinositol turnover and increases the cellular content of 1,2‐diacylglycerol in confluent resting swiss 3T3 cells

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Abstract
Prostaglandin F2α(PGF2α); which stimulates DNA synthesis in resting 3T3 cells, also stimulates the incorporation of [32P]PO4 into phosphatidylinositol. The effect is selective for PGF2α when compared with PGE1, PGE2, and PGF2α. Epidermal growth factor (EGF) also stimulates DNA synthesis but does not affect phosphatidylinositol turnover. PGE1, which acts synergistically with PGF2α to enhance DNA synthesis, does not affect the ability of PGF2α to enhance the incorporation of [32P]PO4 into phosphatidylinositol. PGF2α also causes a small increase in the cellular content of 1,2-diacylglycerol. This effect is not shared by EGF or PGE1. Stimulation of phosphatidylinositol metabolism resulting in an increase in the cellular content of 1,2-diacylglycerol may thus constitute an event in the pathway leading to the initiation of DNA synthesis in which PGF2α differs in its action from EGF.