CHARACTERIZATION OF ANTIGENS FROM ASPERGILLUS-FUMIGATUS .4. EVALUATION OF COMMERCIAL AND EXPERIMENTAL PREPARATIONS AND FRACTIONS IN THE DETECTION OF ANTIBODY IN ASPERGILLOSIS

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Abstract
Commercially available preparations of A. fumigatus, experimental extracts and fractions of A. fumigatus and extracts of 5 other aspergilli were evaluated in a blind manner for their abilities to detect antibody in 66 serum specimens from patients with aspergilloma, allergic bronchopulmonary and invasive aspergillosis and unconfirmed aspergillosis. Rocket immunoelectrophoresis was more sensitive than immunodiffusion in 2 dimensions. Although qualitative differences in antigen composition could be detected, commercial preparations compared favorably with the best experimental extracts in detecting positive specimens. Extracts from young, actively-growing mycelia were most effective and produced the largest numbers of precipitin bands. In contrast to all unfractioned preparations, several fractions of A. fumigatus were devoid of substances that react with C-reactive protein of sera, yet were as effective as the best preparations in detecting sera positive for Aspergillus.

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