An alternative protocol for DNA extraction from formalin fixed and paraffin wax embedded tissue
Open Access
- 27 July 2005
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 58 (8) , 894-895
- https://doi.org/10.1136/jcp.2004.021352
Abstract
Background: DNA extraction from paraffin wax embedded tissue requires special protocols, and most described methods report an amplification success rate of 60–80%. Aims: To propose a simple and inexpensive protocol consisting of xylene/ethanol dewaxing, followed by a kit based extraction. Method: Xylene/ethanol dewaxing was followed by a long rehydration step and a kit based DNA extraction step. Results: This method produced a 100% amplification success rate for fragments of 121 to 227 bp for tamponated formalin fixed paraffin wax embedded tissue. Conclusion: This cost effective and non-laborious protocol can successfully extract DNA from tamponated formalin fixed paraffin wax embedded tissue and should facilitate the molecular analysis of a large number of archival specimens in retrospective studies.Keywords
This publication has 7 references indexed in Scilit:
- Comparison of methods for DNA extraction from paraffin-embedded tissues and buccal cellsCancer Detection Prevention, 2003
- Detection of Microsatellite Instability by Real Time PCR and Hybridization Probe Melting Point AnalysisLaboratory Investigation, 2001
- Assessment of Clonality in Cutaneous Lymphoid Infiltrates by Polymerase Chain Reaction Analysis of Immunoglobulin Heavy Chain Gene RearrangementAmerican Journal of Clinical Pathology, 1997
- B-cell gene rearrangement in benign and malignant lymphoid proliferations of mucosa-associated lymphoid tissue and lymph nodesHuman Pathology, 1997
- Detection of immunoglobulin gene rearrangement of B cell non-Hodgkin's lymphomas and leukemias in fresh, unfixed and formalin-fixed, paraffin-embedded tissue by polymerase chain reaction.1993
- SAMPLE PREPARATION FROM PARAFFIN-EMBEDDED TISSUESPublished by Elsevier ,1990