Incorporation of label from d-β-hydroxy-[14C]butyrate and [3-14C]acetoacetate into amino acids in rat brain in vivo
- 1 April 1971
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 122 (2) , 135-138
- https://doi.org/10.1042/bj1220135
Abstract
The metabolism of ketone bodies by rat brain was studied in vivo. Rats starved for 48h were given either d-beta-hydroxy[3-(14)C]butyrate or [3-(14)C]acetoacetate by intravenous injection and killed after 3 or 10min. Total radioactivity in the acid-soluble material of the brain and the specific radioactivities of the brain amino acids glutamate, glutamine, aspartate and gamma-aminobutyrate were determined. A group of fed animals were also given d-beta-hydroxy[3-(14)C]butyrate. In the brains of all animals (14)C was present in the acid-soluble material and the specific radioactivity of glutamate was greater than that of glutamine.Keywords
This publication has 18 references indexed in Scilit:
- Uptake of ketone bodies by rat brain in vivoBiochemical Journal, 1971
- Selective inhibition of glucose oxidation by triethyltin in rat brain in vivoBiochemical Journal, 1970
- METABOLISM OF [14C]LEUCINE AND [14C]ACETATE IN SENSORIMOTOR CORTEX, THALAMUS, CAUDATE NUCLEUS AND CEREBELLUM OF THE CATJournal of Neurochemistry, 1968
- CHANGES IN D(-)-BETA-HYDROXYBUTYRIC DEHYDROGENASE ACTIVITY DURING BRAIN MATURATION IN RAT1967
- Utilization in vivo of glucose and volatile fatty acids by sheep brain for the synthesis of acidic amino acidsBiochemical Journal, 1966
- PRECURSORS IN VIVO OF GLUTAMATE, ASPARTATE AND THEIR DERIVATIVES OF RAT BRAINJournal of Neurochemistry, 1966
- THE EFFECT OF PERIODIC CONVULSIONS INDUCED BY 1,1‐DIMETHYLHYDRAZINE ON THE SYNTHESIS OF RAT BRAIN METABOLITES FROM [2‐14C]GLUCOSEJournal of Neurochemistry, 1966
- THE FATE OF THE α‐H ATOM OF L‐LACTATE IN PERFUSED RAT LIVER*Annals of the New York Academy of Sciences, 1965
- AMINO ACID METABOLISM IN RAT BRAIN STUDIED WITH 14C‐LABELLED GLUCOSEJournal of Neurochemistry, 1964
- Enzymic determination of d(−)-β-hydroxybutyric acid and acetoacetic acid in bloodBiochemical Journal, 1962