The Effect of Various colI Factors on the Induction of Phage P22 by Ultraviolet Radiation

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Abstract
The burst size of phage p22 in Salmonella typhimurium strain lt2 is 200 to 1000, whether a clear plaque mutant (1, 2) or a low multiplicity of wild-type (c +) phage is used. However, when vegetative phage growth is induced in strain lt2 lysogenized by c + phage by exposing it to ultraviolet light from a low pressure germicidal lamp, the burst size is far smaller and usually less than 40. This is so even when the prophage is one of the non-excluding mutants already described (3) and the bacteria are superinfected with virulent (c 2) phage just before irradiation, despite the appearance of both c + and c 2 phage in the burst. These observations suggested that the dose of u.v. radiation producing maximal induction damaged the bacteria to a degree that severely limited their capacity to support phage growth.

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