Application of Atomic Absorption Spectrophotometry in the Determination of Iron in Urine

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Abstract
Atomic absorption spectrophotometry is a specific, rapid and convenient method for the determination of iron (Fe) in urine. Analyses can be carried out directly on 1 ml samples of untreated urine. The coefficient of variation is less than 1%. No interferences were seen from a large number of cations, anions, acids, organic substances, and chelators. Urine showed a slight enhancing effect; Fe recoveries in urine ranged from 101-105% when aqueous standards were used. Excesses of salt concentrations and proteins have a mild depressive effect. Atomic absorption spectrophotometry is recommended as the method of choice for the determination of Fe in urine. In a case of Fe poisoning this technique proved to be extremely useful and rapid in monitoring Fe excretion in urine following the therapeutic use of specific chelators.

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