Comparison of various methods for differentiation of staphylococci and micrococci

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Abstract
A modified oxidase test and susceptibility to furazolidone and lysostaphin were evaluated in conjunction with the Staph-Ident strip to accurately differentiate between staphylococci and micrococci. Clinical isolates (414) of catalase-positive, gram-positive cocci were each tested with the Staph-Ident strip and by glucose fermentation, acid production from glycerol, susceptibility to furazolidone and lysostaphin and the oxidase test. Based on the reference methods of glucose fermentation and acid production from glycerol, 396 (95.6%) of the organisms were classified as Staphylococcus sp. and 18 (4.4%) were classified as Micrococcus sp. Of the staphylococci, 99% were oxidase negative and susceptible to furazolidone; 82% were susceptible to lysostaphin. All of the micrococci were oxidase-positive and resistant to furazolidone and lysostaphin. Of the staphylococci, 99% were identified to species by the Staph-Ident strip. Of the micrococci, 6 (33%) were incorrectly identified as Staphylococcus sp. (3 each of S. hominis and S. saprophyticus). Because of the demonstrated sensitivity and specificity of the oxidase and furazolidone susceptibility tests, it is suggested that either of these methods be used in the clinical laboratory to accurately differentiate between staphylococci and micrococci. It is also suggested that when working with the Staph-Ident strip, additional testing such as furazolidone susceptibility or oxidase activity should be performed to provide increased accuracy in the differentiation and characterization of members of the family Micrococcaceae.