Properties of the iron–sulphur proteins of the benzene dioxygenase system from Pseudomonas putida
- 1 February 1979
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 177 (2) , 393-400
- https://doi.org/10.1042/bj1770393
Abstract
A purification procedure was developed to stabilize the iron-sulphur proteins of the benzene dioxygenase system from Pseudomonas putida. The intermediate electron-carrying protein has a mol. wt. of 12300 and possesses one (2Fe–2S) cluster, whereas the terminal dioxygenase has a mol.wt. of 215300 and possesses two (2Fe–2S) clusters. The order and stoicheiometry of electron transfer and of the whole system are described.Keywords
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