Botulinum C3 exoenzyme blocks the tyrosine phosphorylation of p125FAK and paxillin induced by bombesin and endothelin
- 14 November 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 354 (3) , 315-319
- https://doi.org/10.1016/0014-5793(94)01148-6
Abstract
In this study we examined the role of rho p21 in neuropeptide-stimulated tyrosine phosphorylation. Intact Swiss 3T3 cells were treated with the Clostridium botulinum C3 exoenzyme which specifically ADP ribosylates and inactivates rho p21. C3 exoenzyme treatment of cells caused a marked decrease in both bombesin- and endothelin-stimulated tyrosine phosphorylation of multiple proteins, including p125 focal adhesion kinase (FAK) and paxillin. Our results suggest that rho p21 is a component of the signal transduction pathway linking seven transmembrane domain receptors with tyrosine phosphorylation and cytoskeletal eventsKeywords
This publication has 30 references indexed in Scilit:
- Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genesPublished by Elsevier ,2004
- Lysophosphatidic acid induces tyrosine phosphorylation and activation of MAP‐kinase and focal adhesion kinase in cultured Swiss 3T3 cellsFEBS Letters, 1993
- The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factorsCell, 1992
- Paxillin is a major phosphotyrosine-containing protein during embryonic development.The Journal of cell biology, 1991
- Bombesin, vasopressin, and endothelin rapidly stimulate tyrosine phosphorylation in intact Swiss 3T3 cells.Proceedings of the National Academy of Sciences, 1991
- Neuropeptides as cellular growth factors: role of multiple signalling pathwaysEuropean Journal of Clinical Investigation, 1991
- Paxillin: a new vinculin-binding protein present in focal adhesions.The Journal of cell biology, 1990
- A role for neuropeptides in the control of cell proliferationDevelopmental Biology, 1987
- Clostridium botulinum type C produces a novel ADP‐ribosyltransferase distinct from botulinum C2 toxinFEBS Letters, 1987
- Vectors for selective expression of cloned DNAs by T7 RNA polymeraseGene, 1987