A low tech in vitro procedure using faecal liquor for the estimation of digestibility of Forages
- 1 January 1998
- journal article
- programme
- Published by Cambridge University Press (CUP) in Proceedings of the British Society of Animal Science
- Vol. 1998, 59
- https://doi.org/10.1017/s0308229600032724
Abstract
Two-stage techniques Tilley & Terry (1963) for the estimation of digestibility have 3 main disadvantages for use in developing countries with limited laboratory facilities. Carbon dioxide is needed to saturate the buffers; a centrifuge is necessary to separate residues from solubilised materials; and the methods uses pepsin as a proteolytic agent. The aim of the present study was to eliminate these three requirements for the faecal liquor method ( Omed et al., 1989), by replacing bicarbonate with phosphate buffer (Marten & Barnes, 1980), pepsin with biological washing liquid, and centrifugation with sedimentation.Keywords
This publication has 2 references indexed in Scilit:
- A comparison of three laboratory techniques for the estimation of the digestibility of feedstuffs for ruminantsThe Journal of Agricultural Science, 1989
- A TWO‐STAGE TECHNIQUE FOR THE IN VITRO DIGESTION OF FORAGE CROPSGrass and Forage Science, 1963