Generation of Macrophage Variants With 5-Azacytidine: Selection for Mannose Receptor Expression

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Abstract
Mannose receptors are expressed only in primary macrophages. Established macrophage-derived cell lines, although apparently possessing the potential to synthesize mannose receptors, do not express them on their plasma membranes. Using the drug 5-Azacytidine, mannose receptor expression was induced in the macrophage-derived mouse cell line J774. Receptor positive cells were sorted through a fluorescent activated cell sorter (FACS) prior to cloning. Clones were isolated which continuously express mannose receptors in culture. These macrophages were able to endocytose β-glucuronidase and phagocytose yeast particles via mannose receptors. Secretion of the lysosomal enzyme β-hexosaminidase was also reduced in proportion to the degree of mannose receptor expression.

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