The Stimulation of Splenic Foci in Vitro

Open Access
Publisher Website
Google Scholar
Abstract
The “splenic focus” technique has been extended to an analysis of the focal response in vitro to both carrier and haptenic determinants of the 2,4-dinitrophenylhemocyanin (DNP-Hy) molecule. The response was shown to depend on the use of previously immunized donor cells and was directly related to the number of such cells injected. While anti-DNP foci could be stimulated with the hapten on heterologous carriers, the number of foci was maximized by the use of DNP-Hy, the complex used for primary immunization of the donor cells. Foci producing anti-DNP antibody were detected in fragments producing no detectable anti-Hy antibody; however, more fragments were found with foci producing both anti-DNP and anti-Hy antibody than would have been expected if stimulation were by two determinants which were independent of one another.

This publication has 0 references indexed in Scilit: