RAPID AND SIMPLE RADIOACTIVE METHOD FOR DETERMINATION OF DISULFIRAM AND ITS METABOLITES FROM A SINGLE SAMPLE OF BIOLOGICAL FLUID OR TISSUE

Google Scholar
  • 1 January 1977
    • journal article
    • research article
    • Vol. 17  (3) , 481-496
Abstract
An analytical method is described for the determination of radioactive disulfiram, diethyldithiocarbamate, diethyldithiocarbamate-methyl ester, diethyldithiocarbamate-glucuronide, inorganic sulfate, and a protein bound 35S fraction from a single sample of either plasma, urine or tissue. The procedure is based upon quantitative stepwise extraction or precipitation of the individual compounds and is both specific and precise. The applicability of the methods developed for the determination of 35S disulfiram and its 35S metabolites in plasma and urine from a dog given 35S disulfiram i.v., and in mouse brain from mice given 35S disulfiram i.p. are illustrated.