Determination of the translation start site of the large subunit of ribulose-1,5-bisphosphate carboxylase from maize
- 1 January 1984
- journal article
- research article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 3 (6) , 403-406
- https://doi.org/10.1007/bf00033388
Abstract
Sequence studies of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase from maize indicate the presence of two translation start sites, 18 bp apart. Each site is preceded by a suitable ribosome binding region. By using a simplified E. coli-based in vitro system which measures fromation of the first dipeptide of the gene product, we have determined that only the second methionine initiates translation.Keywords
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