Studies on chromosome arrangement in some higher plants. I. Interphase chromosomes in three liliaceous plants.

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Abstract
The mode of the chromosomal arrangement in interphase nuclei was investigated in the root tips of 3 liliaceous plants; Polygonatum lasianthum with large-, or medium-sized chromomsomes (2n = 20), Dianella ensifolia with relatively small-sized chromosomes (2n = 32) and Tofieldia coccinea var. kondoi with small-sized chromosomes (2n = 30). Both the C-banded and the unpressed preparations stained with aceto-orcein were used. After C-banding treatment, the centromeric regions of all the mitotic chromosomes and the regions of the secondary constriction were deeply stained. Most of these C-banded segments also appeared in interphase nuclei. The centromeric regions tended to be located close to the inner surface of the nuclear envelope during the period from telophase to prophase. The relative extent of the distributional range of the centromeric regions in the nucleus, which was considered to extend from the center of the polar region of the nucleus, was nearly constant for each species but different distinctly among the 3 spp. examined, i.e., the relative extent of the distributional range was the smallest in P. lasianthum and the largest in T. coccinea var. kondoi. The mode of the chromosomal arrangement at telophase tended to be maintained during interphase and prophase. The relationship between the relative extent of the distributional range of the centromeric regions in the nucleus and the variability of the relative positions of chromosomes during the mitotic cycle was discussed.