Chromosome mapping in Pseudomonas aeruginosa PAT

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Abstract
A linkage map of P. aeruginosa PAT was derived from the results of conjugation experiments using the plasmads FP2-2, R68, R91-5 and R68.45. FP2-2 and R68 each mobilize the chromosome from single, distinct transfer origins. R91-5 appears to mobilize the chromosome from 2 such origins, and R68.45 utilizes a number of transfer origins. R68 and R91-5 both mobilized the chromosome with a polarity opposite to that by FP2-2. The locations of the transfer origins of these plasmids are such that it was not possible to demonstrate chromosomal circularity by interrupted mating experiments. The available time-of-entry data combined with linkage data from plate mating experiments indicate that the chromosome of P. eruginosa is circular.