Testicular function of actively immunized male rats with LH releasing hormone (LHRH): A possible role of prolactin on regulation of spermatogenesis.

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Abstract
The effect of testosterone propionate (TP) on the recovery of spermatogenesis was compared in 3 types of experimentally induced gonadotropin-deficient rats; hypophysectomized rats, rats actively immunized to LHRH and hypophysectomized and immunized rats bearing a pituitary isograft. To immunize animals to LHRH, deamidated LHRH conjugated with BSA [bovine serum albumin] (LHRH-BSA) was injected intradermally to male rats 4 times at 2-wk intervals and additional booster injections continued once a month. Anti-LHRH titer was the highest on the 12th wk and the titer remained at a relatively high levels thereafter. A remarkable decrease in testicular weight and testosterone production was achieved in 10 wk associated with a drop in serum LH [luteinizing hormone] and FSH levels. In long-term immunized rat (LIM rats) which had been immunized for > 3 mo., testicular atrophy advanced to a similar extent to that in long-term hypophysectomized rats (HX rats). The administration of TP (1 mg/day) s.c. for 30 days restored spermatogenesis in LIM rats. The simultaneous administration of anti-LH and -FSH sera in addition to TP did not affect the restoration of spermatogenesis in LIM rats. The same TP treatment in HX rats failed to restore spermatogenesis. Spermatogenesis was reinitiated after TP treatment if immunized-hypophysectomized rats received a pituitary isograft. Though the serum PRL [prolactin] level in LIM rats was 1/5 of that in normal rats, a 2.5-fold rise in the PRL level was observed after TP treatment. Evidently, PRL is involved in the process of spermatogenesis.