Regional measurements of NO formedin vivoduring brain ischemia
- 1 April 1997
- journal article
- Published by Hindawi Limited in Acta Neurologica Scandinavica
- Vol. 95 (4) , 219-224
- https://doi.org/10.1111/j.1600-0404.1997.tb00102.x
Abstract
Nitric oxide formed in vivo in the rat brain regions of hippocampus, striatum, neocortex and cerebellum was spin trapped and measured ex vivo by cryogenic electron paramagnetic resonance spectroscopy. In non‐ischemic control animals the rate of nitric oxide (NO) formation in the individual brain regions ranged from 15 to 42 pmolg−1min−1. During exposure to global ischemia for 7 min the generation of NO increased in all parts of the brain. In the hippocampus the rate of NO formation during ischemia increased by 6‐fold from a control rate of 19 pmolg−1min−1. This increase was attenuated 47% by pretreatment with the NO synthase antagonist 7‐nitroindazole, whereas pretreatment with the non‐NMDA receptor antagonist NBQX and the Ca2+ channel blocker NS638 did not influence the NO formation. The data show that short‐duration ischemia elicits a significant, NO‐synthase‐dependent formation of NO in all brain regionsKeywords
This publication has 22 references indexed in Scilit:
- Nitric Oxide Signaling in the Central Nervous SystemAnnual Review of Physiology, 1995
- Nitric Oxide Donors Increase Blood Flow and Reduce Brain Damage in Focal Ischemia: Evidence That Nitric Oxide is Beneficial in the Early Stages of Cerebral IschemiaJournal of Cerebral Blood Flow & Metabolism, 1994
- The Complex Role of Nitric Oxide in the Pathophysiology of Focal Cerebral IschemiaBrain Pathology, 1994
- Regulation of the cerebral circulation by endotheliumPharmacology & Therapeutics, 1992
- Effect of Nitric Oxide Blockade by NG-Nitro-l-Arginine on Cerebral Blood Flow Response to Changes in Carbon Dioxide TensionJournal of Cerebral Blood Flow & Metabolism, 1992
- Quantification of nitric oxide in biological samples by electron spin resonance spectroscopyNeuroprotocols, 1992
- Inhibition of Nitric Oxide Synthesis Increases Focal Ischemic Infarction in RatJournal of Cerebral Blood Flow & Metabolism, 1992
- The neuroprotective effect of a nitric oxide inhibitor in a rat model of focal cerebral ischaemiaBritish Journal of Pharmacology, 1992
- Control of coronary vascular tone by nitric oxide.Circulation Research, 1990
- Calcium, Excitotoxins, and Brain DamagePhysiology, 1990