The Accumulation of Phenolic Acids in Tissue Culture Pathogen Combinations of Solanum tuberosum and Phytophthora infestans

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Abstract
SUMMARY: Suspended tissue cultures of R I-resistant Solanum tuberosum infected with race 4 sporangia of Phytophthora infestans developed toxicity in the culture fluid when tested after 10 days against a zoospore suspension of P. infestans. The toxic material was extracted into ether and salicylic, vanillic and p-hydroxybenzoic acids were identified in the toxic fraction by thin-layer chromatography, gas-liquid chromatography and mass spectrometry. Quantitative estimates of the amounts of material present in the toxic fraction allowed a mixture to be made which closely simulated the natural toxic material. Use of pure materials allowed an analysis of the behaviour of the zoospores with variation in dosage.

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