PURIFICATION AND CHARACTERIZATION OF HUMAN LIVER-SPECIFIC MEMBRANE LIPOPROTEIN (LSP)

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 27  (3) , 381-390
Abstract
LSP, which is present in 105,000 g supernatants of human liver, was previously shown to produce a lesion resembling chronic active hepatitis in rabbits immunized with human liver fractions containing this lipoprotein. It was implicated as the principal target antigen involved in the liver cell cytotoxicity exhibited in vitro by lymphocytes from patients with chronic active hepatitis. The organ-specificity and species cross-reactivity of LSP is confirmed. Although very labile, it was prepared in a stable form by gel filtration on Sepharose 6B in a Tris buffer containing 1 mm disodium EDTA. LSP is stable in a borate buffer containing EDTA but is unstable in a number of other buffer systems tested. When prepared by this method it contains approximately 2% albumin as the only detectable contaminant. Gel filtration studies on the apoprotein of LSP revealed that it has an apparent MW of 4 .times. 106-20 .times. 106. SDS[sodium dodecyl sulfate]-polyacrylamide gel electrophoresis indicated that the lipoprotein may be composed of up to 13 sub-units of different molecular sizes.