EFFECT OF 2,4-DICHLOROPHENOXYACETIC ACID ON PROTEOLYTIC ACTIVITY OF RED KIDNEY BEAN PLANTS

Open Access
Publisher Website
Google Scholar
Abstract
The proteolytic activity of the leaf, stem and root tissue of red kidney bean plants treated with 2, 4-dichlorophenoxyacetic acid and non-treated plants was determined using hemoglobin, glycylglycine, L-cystinyldiglycine, L-cystinyldiglycyldiglycine and chloroacetyl-L-tyrosine substrates. With hemoglobin, glycylglycine and L-cystinyldiglycine substrates, the proteolytic activity of the non-treated leaves was almost double that of the treated leaves. The activity of the treated stems was approximately twice the activity of the non-treated stems whereas the proteolytic activity of the treated roots was slightly less than the control roots. Enzyme prepns. of leaf, stem and root tissue did not hydrolyze chloroacetyl-L-tyrosine and L-cystinyldiglycyldiglycine. When the N components of the treated and non-treated stem tissues were partially fractionated, the major portion of the increased N content of the treated stems was found in the protein fraction.