Failure to adequately use positive control data leads to poor quality mouse lymphoma data assessments.
Open Access
- 1 May 1999
- journal article
- review article
- Published by Oxford University Press (OUP) in Mutagenesis
- Vol. 14 (3) , 261-263
- https://doi.org/10.1093/mutage/14.3.261
Abstract
The mouse lymphoma L5178Y/TK+/–-3.7.2C mutagenesis assay (MLA) is widely used to identify chemicals that are capable of inducing mutational damage (Mitchell et al., 1997). Mutants detected in the assay form a bimodal distribution based on colony size. More than two decades of research demonstrate that both small and large colony trifluorothymidine-resistant (TFTr) colonies are `true mutants' and that the assay detects the broad spectrum of genetic damage known to be involved in the etiology of cancer (Moore-Brown et al., 1981; Moore et al., 1985b; Applegate et al., 1990; Hozier et al., 1992). Because the assay detects mutagens that act through a variety of mechanisms, the MLA is specifically recommended by regulatory agencies for inclusion in hazard identification screening for environmental chemicals and pharmaceuticals (Dearfield et al., 1991; ICH3, 1996).Keywords
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