Reconstitution of Outer Membrane Protein Assembly from Purified Components
- 14 May 2010
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 328 (5980) , 890-892
- https://doi.org/10.1126/science.1188919
Abstract
Bring Out the β-Barrel: The assembly of β-barrel membrane proteins, which are found in the outer membrane of Gram-negative bacteria and in the mitochondria and chloroplasts of eukaryotes, is poorly understood. Now Hagan et al. (p. 890 , published online 8 April; see the Perspective by Stroud et al. ) describe the development of a reconstituted system that recapitulates the process of assembly of β-barrel membrane proteins by the Escherichia coli Bam complex. The assembly of a protein substrate required the purified five-protein Bam complex and several subcomplexes and a chaperone, but did not require an external input of energy.Keywords
This publication has 32 references indexed in Scilit:
- The Periplasmic Molecular Chaperone Protein SurA Binds a Peptide Motif That Is Characteristic of Integral Outer Membrane ProteinsPublished by Elsevier ,2003
- Evolutionary conservation of biogenesis of β-barrel membrane proteinsNature, 2003
- The versatile β-barrel membrane proteinCurrent Opinion in Structural Biology, 2003
- Machinery for protein sorting and assembly in the mitochondrial outer membraneNature, 2003
- Role of a Highly Conserved Bacterial Protein in Outer Membrane Protein AssemblyScience, 2003
- Genetic Evidence for Parallel Pathways of Chaperone Activity in the Periplasm of Escherichia coliJournal of Bacteriology, 2001
- Genetic Basis for Activity Differences Between Vancomycin and Glycolipid Derivatives of VancomycinScience, 2001
- The assembly pathway of outer membrane protein PhoE of Escherichia coliEuropean Journal of Biochemistry, 2000
- The purified E. coli integral membrane protein is sufficient for reconstitution of SecA-dependent precursor protein translocationCell, 1990
- Assembly of translocation-competent proteoliposomes from detergent-solubilized rough microsomesCell, 1990