The Pto kinase conferring resistance to tomato bacterial speck disease interacts with proteins that bind a cis-element of pathogenesis-related genes
Open Access
- 1 June 1997
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 16 (11) , 3207-3218
- https://doi.org/10.1093/emboj/16.11.3207
Abstract
In tomato, the Pto kinase confers resistance to bacterial speck disease by recognizing the expression of a corresponding avirulence gene, avrPto , in the pathogen Pseudomonas syringae pv. tomato . Using the yeast two‐hybrid system, we have identified three genes, Pti4 , Pti5 and Pti6 , that encode proteins that physically interact with the Pto kinase. Pti4/5/6 each encode a protein with characteristics that are typical of transcription factors and are similar to the tobacco ethylene‐responsive element‐binding proteins (EREBPs). Using a gel mobility‐shift assay, we demonstrate that, similarly to EREBPs, Pti4/5/6 specifically recognize and bind to a DNA sequence that is present in the promoter region of a large number of genes encoding ‘pathogenesis‐related’ (PR) proteins. Expression of several PR genes and a tobacco EREBP gene is specifically enhanced upon Pto–avrPto recognition in tobacco. These observations establish a direct connection between a disease resistance gene and the specific activation of plant defense genes.Keywords
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