Serial Cultivation of Human Leukemic Cells.

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Abstract
Summary Serial suspension cell cultures have been established from the peripheral blood of 3 out of 3 patients with high-count acute lymphoblastic or myeloblastic leukemia. A period of latency from 4 to 10 weeks, during which the cultures were regularly refed, preceded the start of multiplication. The techniques for initiation and maintenance of the cultures are described as well as observations made during these procedures. Preliminary attempts at virus isolation from all of the cultures were negative. Evidence suggesting a possible latent virus infection in one of the serial cultures is presented.