Factors affecting measurement of total alkaline phosphatase activity in human serum, especially wavelength accuracy.
- 1 June 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 24 (6) , 938-941
- https://doi.org/10.1093/clinchem/24.6.938
Abstract
Serum alkaline phosphatase estimations by the Selected Method [Clin. Chem. 21, 1988 (1975)] are very sensitive to small errors in the wavelength setting of the spectrophotometer. With a setting error as small as 3 nm, results can be too low by as much as 23%. The product of the reaction, 4-nitrophenol, can be prepared in very pure form and can be used to check both the wavelength and absorbance accuracy of a spectrophotometer. The molar absorptivity for 4-nitrophenol obtained in the instrument being used should be used in calculating alkaline phosphatase activities.This publication has 2 references indexed in Scilit:
- 4-Nitrophenol in 4-nitrophenyl phosphate, a substrate for alkaline phosphatase, as measured by paired-ion high-performance liquid chromatography.Clinical Chemistry, 1977
- Temperature dependence of the absorbance of alkaline solutions of 4-nitrophenyl phosphate--a potential source of error in the measurement of alkaline phosphatase activity.Clinical Chemistry, 1977