Recombination genes on the Escherichia coli sex factor specific for transposable elements.
- 1 May 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (5) , 2814-2818
- https://doi.org/10.1073/pnas.77.5.2814
Abstract
The E. coli sex factor stimulates precise excision of transposons Tn5 and Tn10 from sites either within the bacterial chromosome or within the factor itself. Two kinds of mutations that affect this activity were isolated. The ferA mutations eliminate the stimulation; the ferB mutations enhance it in the presence of ferA+. Apparently, ferA defines a sex factor gene that stimulates precise excision. The ferB mutations also specifically increase the rate of recombination between 2 IS3 [insertion sequence] elements on F'' lac-pro (F''128) in a reaction that requires the product of recA. The stimulation of this recombination by ferB also requires an active ferA gene, which implies that the ferA gene stimulates this reaction and precise excision. A ferA mutation was mapped at 84.2 kilobases [kb] on the F factor, and a ferB mutation was mapped at 82.5 kb. The fer mutants were obtained by an approach that permits the isolation of mutants affecting precise excision.Keywords
This publication has 18 references indexed in Scilit:
- Physical structures of Tn10-promoted deletions and inversions: Role of 1400 bp inverted repetitionsCell, 1979
- Inversions and deletions of the Salmonella chromosome generated by the translocatable tetracycline resistance element Tn10Journal of Molecular Biology, 1979
- In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites.Proceedings of the National Academy of Sciences, 1978
- Conjugation proteins encoded by the F sex factorNature, 1977
- Excision of F plasmid sequences by recombination at directly repeated insertion sequence 2 elements: involvement of recA.Proceedings of the National Academy of Sciences, 1977
- The sequence organization of the integrated F plasmid in two Hfr strains of Escherichia coliJournal of Molecular Biology, 1976
- Acquisition of a determinant for chloramphenicol resistance by coliphage lambda.Proceedings of the National Academy of Sciences, 1975
- Mutagenesis by insertion of a drug-resistance element carrying an inverted repetitionJournal of Molecular Biology, 1975
- Isolation of polar insertion mutants and the direction of transcription of ribosomal protein genes in E. coliNature, 1975
- Plasmid identification using specific endonucleasesMolecular Genetics and Genomics, 1974