Surveyor Substrates: Energy-Transfer Gauges of Active Center Topography during Catalysis
- 1 November 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 67 (3) , 1383-1389
- https://doi.org/10.1073/pnas.67.3.1383
Abstract
An approach is described for the simultaneous measurement of catalysis, the distance from an active-site moiety to fluorescent substrate moieties of an enzyme substrate complex, and the microenvironment of such substrate groups. It is illustrated by means of a three-component energy transfer relay system, consisting of cobalt carboxypeptidase, its fluorescent dansylated peptide substrates, and tryptophanyl residues of the enzyme. The mode of procedure can be applied generally to yield conjoint information on functional and structural aspects of active centers of enzymes.Keywords
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