IMMUNOGENIC ACTIVITY OF A CELL-WALL FRACTION EXTRACTED FROM BRUCELLA-ABORTUS IN GUINEA-PIGS

  • 1 January 1978
    • journal article
    • research article
    • Vol. B129  (4) , 571-579
Abstract
A cell wall fraction (F8) extracted by boiling sodium dodecyl sulfate at 4% from B. abortus 99S was used with oil adjuvant to vaccinate groups of 10 guinea pigs, at doses equivalent to 1 .times. 109 and 1 .times. 1010 bacteria, once or twice at 3 mo. intervals. H38 vaccine, a total cell vaccine from formalized B. melitensis 53 H38, was used as a reference at doses 3 .times. 108 and 3 .times. 109 bacteria. These doses have about the same vaccinal activity in mice being, respectively, equal to 10 and 100 mouse optimal dose (MOD). One extra group of guinea pigs received 2 injections of 100 .mu.g of smooth lipopolysaccharide (LPS-S) of B. melitensis 16M in adjuvant. The control group received the adjuvant only. Guinea pigs were challenged 3 mo. after the last vaccination with 5000 colony-forming units of B. abortus 544 and autopsied 40 days later. The spleen and 8 lymph nodes were cultured; a guinea pig was considered protected if no Brucella was found in any sample. Protection afforded by the 2 vaccines was dose-dependent. H38 vaccine gave better protection (infected 24%) than F8 (46%) since a higher dose was needed to obtain the same level of protection: i.e., 100 MOD of F8 was about equal to 10 MOD of H38 (35 and 37%, respectively). Recall did not improve immunity and LPS-S did not vaccinate at all.

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