A NEW LARGE CHYLOMICRON REMNANT FROM CHOLESTEROL‐FED RABBITS

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Abstract
The lipoproteins of density < 1 063 g/ml of cholesterol‐fed rabbits were subjected to analytical ultracentrifugation. and many rabbits two peaks were found in the very low density (Sf > 20) portion of the lipoprotein spectrum. They were isolated by preparative ultracentrifugation and analysed. The smaller particles (remnant chylomicrons) had a peak Sf of 37, mean diameter of 36 nm. mean density of 1·00 g/ml, and their chemical composition agreed closely with previous reports. The larger particles had a peak Sf of 270, mean diameter of 80 nm. mean density of 0·97 g/ml and a high (80%) cholesterol ester and low (4%) triglyceride content. The fatty acid composition of the cholesterol esters, phospholipids and triglycerides was similar in both fractions. It is proposed that these law lipoprotein particles are also remnant chylomicrons. Possible reasons are presented to explain the presence of this second peak in the very low density lipoprotein spectrum.