Biochemically Marked Lymphocytoid Lines: Establishment of Lesch-Nyhan Cells
- 2 October 1970
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 170 (3953) , 89-90
- https://doi.org/10.1126/science.170.3953.89
Abstract
Two lymphocytoid cell lines have been established from a patient with the Lesch-Nyhan syndrome. The cells are deficient in hypoxanthine-guanine phosphoribosyltransferase, as demonstrated by their failure to incorporate [H3]hypoxanthine and by their inability to grow in medium in which they were nutritionally dependent upon exogenous hypoxanthine. This represents the first establishment of presumptively permanent human lymphocytoid cell lines that are deficient in a specific enzyme.Keywords
This publication has 10 references indexed in Scilit:
- Cloning Human Lymphocytes in vitroNature, 1970
- Human hemapoietic cell lines: A progress reportIn Vitro Cellular & Developmental Biology - Plant, 1969
- Lymphocytoid Lines from Persons with Sex Chromosome AnomaliesScience, 1969
- X-Linked Hypoxanthine-Guanine Phosphoribosyl Transferase Deficiency: Heterozygote Has Two Clonal PopulationsScience, 1968
- Infectious Mononucleosis: Continuous Suspension Culture of Peripheral Blood LeucocytesNature, 1968
- Establishment of Cell Lines from Peripheral Leucocytes in Infectious MononucleosisNature, 1967
- Culture of human leukemia cellsCancer, 1966
- Continuous culture of human lymphoblasts from peripheral blood of a child with acute leukemiaCancer, 1965
- GENETICS OF HUMAN CELL LINES, IV. DNA-MEDIATED HERITABLE TRANSFORMATION OF A BIOCHEMICAL TRAITProceedings of the National Academy of Sciences, 1962