Purification of the oligosaccharide-cleaving enzymes of Flavobacterium meningosepticum
- 1 June 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Glycobiology
- Vol. 1 (3) , 257-263
- https://doi.org/10.1093/glycob/1.3.257
Abstract
Four oligosaccharide chain-cleaving enzymes, including two new endoglycosidases distinct from endo-β-acetylglu-cosaminidase (Endo) F1, have been identified and purified to homogeneity from cultural filtrates of Flavobacterium meningosepticum. FPLC-directed hydrophobic-interaction chromatography in conjunction with high-resolution ion-exchange chromatography provided a more simple, rapid method for the isolation of endoglycosidase F1, F2 and F3, and the amidase, peptide-N4-N-acetyl-β-D-glucosaminyI)-asparagine amidase (PNGase F), in >50% yield. The specificity of PNGase F and Endo F1 are well established. Endo F2 and Endo F3 represent new distinct endoglycosidases that prefer complex as compared to high-mannose asparagine-linked glycans. Endo F2 cleaved biantennary oligosaccharides, whereas Endo F3 cleaved both bi- and triantennary oligosaccharides.Keywords
This publication has 0 references indexed in Scilit: