The Inorganic Phosphate and a Labile Form of Organic Phosphate in the Gastrocnemius of the Frog

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Abstract
Estimation of the inorganic phosphate in resting voluntary muscles must be done without the use of acid solutions owing to the presence of a phosphoric ester which is rapidly hydrolyzed by acid. Of the supposed "inorganic phosphate" of the resting frog''s gastrocnemius 70-80% is organic phosphate of this labile nature ("phosphagen"). In fatigue most, and in rigor all, of the phosphagen is broken down with the liberation of inorganic phosphates.

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