Cloning a synthetic gene for human stefin B and its expression in E. coli
- 19 October 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 239 (1) , 41-44
- https://doi.org/10.1016/0014-5793(88)80541-6
Abstract
A gene coding for human stefin B was synthesized by the solid-phase phosphite method and cloned in the pUC8 cloning vector. The insert with the verified DNA sequence was subcloned into two expression vectors and expressed in E. coli as a fusion protein with β-galactosidase and as a native protein. The CNBr cleaved fusion protein and the native recombinant stefin B were inhibitory to papain and reacted with antibodies against human stefin B.Keywords
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