Sequence-Specific Isotope Effects on the Cleavage of DNA by Bleomycin
- 22 September 1989
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 245 (4924) , 1396-1399
- https://doi.org/10.1126/science.2476851
Abstract
Bleomycin is a metal- and oxygen-dependent DNA cleaver. The chemistry of DNA damage has been proposed to involve rate-limiting abstraction of the 4'-hydrogen. A DNA fragment has been prepared that contains [4'-2H]thymidine residues of high isotopic content. Primary kinetic isotope effects have been directly observed at individual thymidine residues with DNA sequencing technology.Keywords
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