Using DEAE-cellulose two distinct β-glucosidase enzymes have been identified in white clover; one form is primarily associated with the seed, the other with young leaves. Evidence is presented for the separate nature of β-glucosidase and β-galactosidase activity in dry seed flour and in young leaf tissue. These results are considered in relation to the function of the Li locus in white clover, which is shown to control both β-glucosidase and β-galactosidase activity in expanding leaves.