Propargylglycine as a Fungal Inhibitor: Effect on Sulphur Amino Acid Metabolism

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Abstract
~~~~~ ~ ~ The effect of propargylglycine (PPG) on four fungal species was studied. y-Cystathionase was the major target of the antibiotic, as in mammalian but not in bacterial cells. Highly PPG- sensitive y-cystathionase was found in Aspergillus nidulans and Neurospora crassa, less sensitive in Saccharomyces cerevisiae and insensitive in Cephalosporium acremonium. The effects of y- cystathionase inhibition on growth depend on the role this enzyme plays in cysteine synthesis. Inhibition by PPG was reversed by cysteine, and also by other amino acids at high concentrations. The results of transport assays of wild-type strains and PPG-resistant mutants indicated that propargylglycine was taken up by amino acid permeases in A. nidulans and S. cerevisiae.

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