Rational design and PCR-based synthesis of an artificialSchizophyllum communexylanase gene
Open Access
- 1 January 1993
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 21 (21) , 4923-4928
- https://doi.org/10.1093/nar/21.21.4923
Abstract
A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Threelong oligonucleotides were synthesized and used in combination with flanking PCR primers to generate a 607 base pair gene which contained 31 unique locations for restriction enzyme cleavage. The amino acid sequence was tailored for expression in Eschericia coli by using only those codons found in highly expressed E.coli genes. Theavailability of the gene will facilitate analysis of the structure and function of this and other β-(1,4) xylanases.Keywords
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