Human nuclear protein interacting with a conservative sequence motif of Alu‐family DNA repeats

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Abstract
Human retrotransposons, Alu‐family DNA repeats (AFRs), have variable nucleotide sequence but conservative short elements, which may have important functions, are also present. In our previous reports we have described human nuclear DNA‐binding protein interacting with AFRs and evidence was presented that the protein recognizes sequence motif 5′‐GGAGGC‐3′ which is conserved in the spacer of RNA polymerase III promoter of AFRs and in the SV40 T‐antigen‐dependent replication origin of AFRS. In this study it was found that double‐stranded synthetic oligonucleotides containing indicated conservative sequences of AFRs actually have high‐affinity binding site for HeLa nuclear protein. The data suggest that non‐infected human cells contain nuclear DNA‐binding protein which recognizes the conservative sequence motif of AFRs — GGAGGC.