Identification of a cis-acting element within the herpesvirus saimiri ORF 6 promoter that is responsive to the HVS.R transactivator.

1 June 1997

journal article
Published by Microbiology Society in Journal of General Virology

Vol. 78 (6) , 1411-1415
https://doi.org/10.1099/0022-1317-78-6-1411

Abstract

We have previously demonstrated that two distinct transcripts are produced from ORF 50, the major transcriptional activating gene of herpesvirus saimiri. The products of these transcripts trans-activate the delayed-early ORF 6 promoter, though to different degrees. Deletion analysis demonstrated that the ORF 50 responsive elements are contained in a 132 bp fragment situated 127-259 bp from the transcription initiation site within the ORF 6 promoter. This fragment conferred ORF 50-responsiveness on an enhancerless simian virus 40. Gel retardation analysis further mapped the responsive elements to a 38 bp fragment.

Keywords

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