A Membrane for the Rapid Concentration of Dilute Protein Samples in an Ultrafilter

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Abstract
The preparation of an anisotropic membrane which retains all proteins of a molecular weight of 25,000 and larger is described. It permits, with the help of appropriate ultrafiltration cells, the 400-fold concentration of 400 ml of a dilute protein solution within a few hours, avoiding all denaturation. Ultrafiltration with this membrane has been compared with a tannic acid precipitation method for the concentration of very dilute serum protein solutions. The ultrafiltration procedure resulted in a more representative recovery of the proteins that were present in the original undiluted sample, with quite fair yields. Some results are given on the proteins found in urine samples from normals and patients with disease.

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