Determination of the Caffeine Metabolite AFMU in Human Urine by Column Switching HPLC

Abstract
A column switching HPLC procedure is described for the quantification of the caffeine metabolite AFMU (5-acetyl amino-6-formylamino-3-methyl uracil) from other metabolites in human urine. The procedure is simple, easy to use and allows the reliable quantification of AFMU without interference from other metabolites.

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