Molecular characterization of Escherichia coli FtsE and FtsX
Open Access
- 1 February 1999
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 31 (3) , 983-993
- https://doi.org/10.1046/j.1365-2958.1999.01245.x
Abstract
The genes ftsE and ftsX are organized in one operon together with ftsY. FtsY codes for the receptor of the signal recognition particle (SRP) that functions in targeting a subset of inner membrane proteins. We have found no indications for a structural relationship between FtsE/X and FtsY. Evidence is presented that FtsE and FtsX form a complex in the inner membrane that bears the characteristics of an ATP‐binding cassette (ABC)‐type transporter. FtsE is a hydrophilic nucleotide‐binding protein that has a tendency to dimerize and associates with the inner membrane through an interaction with the integral membrane protein FtsX. An FtsE null mutant showed filamentous growth and appeared viable on high salt medium only, indicating a role for FtsE in cell division and/or salt transport.Keywords
This publication has 42 references indexed in Scilit:
- Protein secretion by Gram-negative bacterial ABC exporters – a reviewGene, 1997
- Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particleFEBS Letters, 1996
- SecA, the peripheral subunit of the Escherichia coli precursor protein translocase, is functional as a dimerBiochemistry, 1993
- ABC Transporters: From Microorganisms to ManAnnual Review of Cell Biology, 1992
- SecA, an essential component of the secretory machinery of Escherichiacoli, exists as homodimerBiochemical and Biophysical Research Communications, 1991
- The P-loop — a common motif in ATP- and GTP-binding proteinsTrends in Biochemical Sciences, 1990
- The identification of the Escherichia coli fts Y gene product: an unusual proteinMolecular Microbiology, 1990
- Site-directed mutagenesis by overlap extension using the polymerase chain reactionGene, 1989
- Optimal posttranslational translocation of the precursor of PhoE protein across Escherichia coli membrane vesicles requires both ATP and the protonmotive forceBiochimica et Biophysica Acta (BBA) - Biomembranes, 1987
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976