COMPARATIVE RELEASE OF ALKALINE-PHOSPHATASES FROM SERRATIA-MARCESCENS BY OSMOTIC SHOCK AND POLYMYXIN-B TREATMENT

  • 1 January 1976
    • journal article
    • research article
    • Vol. 15  (61-6) , 153-164
Abstract
The comparative release of periplasmic enzymes and proteins from 2 strains of S. marcescens by osmotic shock and polymyxin B treatment was studied. There were significant qualitative and quantitative differences in the materials released by these 2 techniques. The osmotic shock procedure released a higher level of alkaline phosphatase [EC 3.1.3.1] activity and a greater number of protein components than the polymyxin B treatment. The MW of the active components released by the 2 techniques were 190,000 .+-. 10,000 (A''), 140,000 .+-. 10,000 (A) and 110,000 .+-. 10,000 (B) daltons. Components released by polymyxin B were also released by osmotic shock. The reverse was not true. Component B in the osmotic shock fluids was by far the most active. The differences in the release mechanisms of the 2 techniques were discussed. Polymyxin B treatment is the method of choice because of its selectiveness and mildness, despite the rather low level of activity of alkaline phosphatase released.

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