SUSCEPTIBILITY OF XERODERMA PIGMENTOSUM CELLS TO TRANSFORMATION BY MURINE AND FELINE SARCOMA-VIRUSES

  • 1 January 1976
    • journal article
    • research article
    • Vol. 36  (9) , 3294-3299
Abstract
Among various strains of skin fibroblasts tested, 2 strains derived from xeroderma pigmentosum (XP) patients (ages 19 and 25) with neurological complications and 2 strains obtained from heterozygotes (ages 54 and 18) showed relatively higher susceptibility than normal age matched controls to transformation by feline sarcoma virus (FSV). Only 1 strain from a normal individual also showed high susceptibility. There was a parallelism in susceptibilities to FSV and Kirsten murine sarcoma virus (KiMSV). Cells from normal individuals of 46 yr or older exhibited high ratios of FSV:KiMSV titers which were due to their lower susceptibility to KiMSV. Cells from 2 XP patients (ages 25 and 22) and a heterozygote (age 18), who were in a younger age group, manifested such a differential susceptibility to FSV and KiMSV. There was a correlation between the relative sensitivity of XP cells to the cytotoxic effect of 4-nitroquinoline-1-oxide and killing effect of UV light. Pretreatment of fibroblasts from 3 XP patients by a subtoxic dose of 4-nitroquinoline-1-oxide 24 h before viral infection facilitated transformation by KiMSV and FSV, whereas no such effect was observed with 3 normal cell strains similarly treated.

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