HeLa cell messenger RNAs of widely differing sizes encode certain heat shock proteins in theMrregion of 72 000-74 000
- 16 August 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 145 (1) , 57-61
- https://doi.org/10.1016/0014-5793(82)81206-4
Abstract
Cloned cDNA sequences coding for specific 72 000–74 000 M r HeLa cell heat shock proteins (HSPs) have been used as probes to examine cytoplasmic and nuclear RNA of heat shocked HeLa cells. The mRNAs for these HSPs vary widely in size. Whilst the β‐, δ‐ and ε‐HSPs are encoded in mRNAs of 1.9 kb, the γ‐HSP arises from an mRNA three times that size. A search for putative nuclear precursors to these mRNAs revealed high M r candidates at 15.8 kb which accumulate 1–2 h after the initial heat shock, thus supporting the notion that transcriptional control is important in the initial heat shock response.Keywords
This publication has 9 references indexed in Scilit:
- Molecular cloning of cDNA sequences coding for the major (β-, γ-, δ- and ϵ-) heat-shock polypeptides of HeLa cellsGene, 1981
- The Pattern of Protein Synthesis Induced by Heat Shock of HeLa CellsEuropean Journal of Biochemistry, 1981
- Primary sequence of the 5′ flanking regions of the Drosophila heat shock genes in chromosome subdivision 67BNucleic Acids Research, 1981
- Polypeptides encoded by polyadenylated and non-polyadenylated messenger RNAs from normal and heat shocked HeLa cellsNucleic Acids Research, 1981
- The transforming genes of SV40 and polyomaNature, 1979
- [15] Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paperPublished by Elsevier ,1979
- Transcription of structural and intervening sequences in the ovalbumin gene and identification of potential ovalbumin mRNA precursorsCell, 1978
- Identification of a large preculsor of vitellogenin mRNA in the liver of estradiol-treated chicksNucleic Acids Research, 1978
- Methylmercury as a reversible denaturing agent for agarose gel electrophoresisAnalytical Biochemistry, 1976