Light scatter based lymphocyte gate–helpful tool or source of error?
- 1 January 1994
- Vol. 15 (1) , 87-89
- https://doi.org/10.1002/cyto.990150115
Abstract
During flow cytometric analysis of 29 samples of human peripheral blood mononuclear cells, we observed that a light scatter‐based lymphocyte gate would fail to cover all TCR (CD3)‐expressing cells. A mean of 7.9 ± 5.3% of all CD3+ cells was found outside the gate. In addition, this shifted cell subset showed an altered CD4/CD8 ratio (8.1 ± 5.0% of all CD4+ and 13.7 ± 5.8% of all CD8+ cells). The use of standard light scatter‐based lymphocyte gates in acquisition and/or analysis of flow cytometry (FCM) data may thus lead to erroneous results, particularly if T cells are to be studied.Keywords
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