CELL-GROWTH INHIBITORY EFFECT OF INTERFERON - STUDIES OF A RESISTANT CELL-SUBLINE

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 24  (2) , 125-137
Abstract
Studies performed in the CSV subline of mouse L cells suggest that activated endogenous and/or exogenous viral infection might be the factor that modifies the cell surface and, in consequence, the sensitivity of the cell to the cell-growth inhibitory action of interferon. In contrast with the parental L cells, the CSV subline obtained by prolonged passage of L cells in the presence of interferon shows an altered EM morphology, absence of C type particles and a decreased agglutinability with concanavalin A. It is resistant to the cell-growth inhibitory effect of interferon but retains the sensitivity toward its antiviral effect. The sensitivity of the CSV subline toward the cell-growth inhibitory effect of interferon increased significantly after treatment with 5-iododeoxyuridine, infection with the Harvey strain of mouse sarcoma virus and/or prolonged passages in vitro. In this respect, the CSV subline resembles the primary mouse embryonic cells. Since sensitivity of CSV cells increased also after treatment with cyclic AMP and/or prostaglandin E2, it is possible that the cell-growth inhibitory effect of interferon is mediated through the 2nd messenger system.

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